Nageen Sharma
Craniosacral therapy
Faisalabad, Punjab, Pakistan
Years of experience 3

Total years in practice: 3

Published Date
December 31, 2014
Abstract Authors
Shou-Lun Lee, Ting-Yu Chin, Ssu-Chieh Tu, Yu-Jie Wang, Ya-Ting Hsu, Ming-Ching Kao, Yang-Chang Wu
Abstract Source
Evid Based Complement Alternat Med. 2015 ;2015:126302. Epub 2015 Jun 11. PMID: 26170870
Abstract Affiliation
Shou-Lun Lee
Study Type
Research
Conditions
Obesity, Weight Problems
Therapies
Functional Medicine, Holistic Medicine, Naturopathic Medicine, Nutrition, Integrative Medicine, Diet Therapy
Reference
Abstract Content
Background. Purple sweet potato leaves (PSPL) are widely grown and are considered a healthy vegetable in Taiwan. PSPL contain a high content of flavonoids, and the boiling water-extracted PSPL (PSPLE) is believed to prevent metabolic syndrome. However, its efficacy has not yet been verified. Therefore, we investigated the effect of PSPLE on adipocytes. Methods. The differentiated 3T3-L1 cells used in this study were derived from preadipocytes that were differentiated into adipocytes using an adipogenic agent (insulin, dexamethasone, and 3-isobutyl-1-methylxanthine); approximately 90% of the cells were differentiated using this method. Results. Treating the differentiated 3T3-L1 cells with PSPLE caused a dose-dependent decrease in the number of adipocytes rather than preadipocytes. In addition, treatment with PSPLE resulted in apoptosis of the differentiated 3T3-L1 cells as determined by DAPI analysis and flow cytometry. PSPLE also increased the expression of cleaved caspase-3 and poly ADP-ribose polymerase (PARP). Furthermore, PSPLE induced downregulation of interleukin-6 (IL-6) and tumor necrosis factor-? (TNF-?) gene expression in the differentiated 3T3-L1 cells. Conclusions. These results suggest that PSPLE not only induced apoptosis but also downregulated inflammation-associated genes in the differentiated 3T3-L1 cells.
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