Nageen Sharma
Craniosacral therapy
Faisalabad, Punjab, Pakistan
Years of experience 3

Total years in practice: 3

Published Date
December 31, 2015
Abstract Authors
L Zhang, Z K Zhang, S Liang
Abstract Source
Genet Mol Res. 2016 ;15(2). Epub 2016 Jun 10. PMID: 27323164
Abstract Affiliation
L Zhang
Study Type
Diabetic Retinopathy
Functional Medicine, Naturopathic Medicine
Abstract Content
Diabetic retinopathy (DR) is a frequent microvascular complication of diabetes, and one of the most common causes of legal blindness in the world. Epigallocatechin-3-gallate (EGCG) produces an anti-oxidative and anti-inflammatory effect against various human s. In this study, we determined the effect of EGCG on a human retinal endothelial cell (HREC) line. The cell viability was determined by a standard MTT assay, while the cell cycle and apoptosis rate were analyzed by flow cytometry. Inflammatory marker expression was detected by enzyme-linked immunosorbent assay. Treatment of HRECs with EGCG (20 and 40 mM) led to a significant decrease in the apoptosis rate (2.35± 0.56 and 1.24 ± 0.32%). The culture supernatant of cells treated with high glucose concentrations showed significantly higher levels of TNF-? (598.7 ± 89.7 vs 193.2 ± 38.5 pg/mL; P
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