Nageen Sharma
Craniosacral therapy
Federal Hostels , Faisalabad, Punjab, Pakistan, 38000
Years of experience 3

Total years in practice: 3

Published Date
May 31, 2015
Abstract Authors
Antonella Di Sotto, Gabriela Mazzanti, Nijole Savickiene, Rasa Staršelskyt?, Vaida Baksenskaite, Silvia Di Giacomo, Annabella Vitalone
Abstract Source
Pharm Biol. 2015 Jun ;53(6):935-8. Epub 2014 Dec 4. PMID: 25473940
Abstract Affiliation
Antonella Di Sotto
Study Type
Therapeutic Substances
Cancer Care
Herbal Therapy, Holistic Medicine
Abstract Content
CONTEXT: Urtica dioica L. (Urticaceae), stinging nettle, has been employed as a folklore remedy for a wide spectrum of ailments, including urinary disorders, prostatic hyperplasia, and liver s. It has been also used traditionally for cancer treatment. OBJECT: To evaluate the potential chemopreventive properties of a protein fraction from the aerial part of Urtica dioica (namely UDHL30). MATERIALS AND
UDHL30 has been tested for the antimutagenic activity in bacteria (50-800?g/plate; Ames test by the preincubation method) and for the cytotoxicity on human hepatoma HepG2 cells (0.06-2 mg/mL; 24 and 48 h incubation). Moreover, the antioxidant activity of UDHL30 (0.1-1200 ?g/mL; ABTS and superoxide-radical scavenger assays) was evaluated as potential protective mechanisms.
UDHL30 was not cytotoxic on HepG2 cells up to 2 mg/mL; conversely, it exhibited a strong antimutagenic activity against the mutagen 2-aminoanthracene (2AA) in all strains tested (maximum inhibition of 56, 78, and 61% in TA98, TA100, and WP2uvrA strains, respectively, at 800?g/plate). In addition, a remarkable scavenging activity against ABTS radical and superoxide anion (IC50 values of 19.9 ± 1.0 ?g/mL and 75.3 ± 0.9 ?g/mL, respectively) was produced. DISCUSSION AND CONCLUSIONS: UDHL30 possesses antimutagenic and radical scavenging properties. Being 2AA a pro-carcinogenic agent, we hypothesize that the antimutagenicity of UDHL30 can be due to the inhibition of CYP450-isoenzymes, involved in the mutagen bioactivation. The radical scavenger ability could contribute to 2AA-antimutagenicity. These data encourage further studies in order to better define the potential usefulness of UDHL30 in chemoprevention.
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