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Immunity research studies for holistic treatments

Around the world over thousands of years, patients have received root-cause holistic treatment for their diseases with personalized
treatment, diet and lifestyle modification recommendations. Read the inspiring true stories of practitioners who heal people and who recovered
from their problems after immunity treatment at their clinics. Many have been generous to share their knowledge and experience for the benefit
of other holistic experts and patients alike. Many practitioners share their Case Studies and the healing powers of immunity and related therapies
as they heal people who benefited from our expertise.

/ title=”In vitro study on the immunological effect of bromelain and trypsin on mononuclear cells from humans.”>
In vitro study on the immunological effect of bromelain and trypsin on mononuclear cells from humans.

August 2005

Background:
Proteolytic enzymes such as bromelain and trypsin are used as an adjuvant therapeutic approach in the treatment of chronic inflammatory, malignant and autoimmune s. In vitro studies have shown that proteolytic enzymes modulate surface adhesion molecules on T-cells. In this study we analysed the influence of bromelain and trypsin on the cytokine production and proliferation of peripheral blood mononuclear cells (PBMC) from patients with a classical cellular mediated autoimmune disorder, namely encephalomyelitis disseminata (ED) as well as from healthy controls. –
METHODS:
PBMC from patients with ED (n=12) and healthy controls (n=12) were cultured for seven days at 37 degrees C under three different conditions: without antigen, with bromelain and with trypsin (final concentrations 10-1000 microg/ml). Proliferation was determined by superset3H-thymidine incorporation. Secretion of cytokines into the supernatant was measured by a double sandwich ELISA. Intracellular cytokines were determined by flow cytometric analysis. –
Results:
PBMC from patients with ED and healthy controls showed a significantly increased proliferative response to bromelain (ED: 14053+/- 7585 cpm with bromelain vs. spontaneous proliferation of 430+/- 255 cpm; healthy controls: 10689+/- 4607 cpm vs. 327+/-193) but not to trypsin. Bromelain induced in all 24 individuals a significant increase of the macro-phage/monocyte associated cytokines interleukin (IL)-6, granulocyte-macrophage-colony stimulating factor (GM-CSF), tumour necrosis factor alpha (TNFa) (p

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/ title=”The effect of oral steroids with and without vitamin D3 on early efficacy of immunotherapy in asthmatic children.”>
The effect of oral steroids with and without vitamin D3 on early efficacy of immunotherapy in asthmatic children.

October 2009

Summary Background The possibility of additional strategies to enhance the effectiveness of specific immunotherapy (SIT) is highly attractive. Aim The aim of our study was to assess the influence of oral corticosteroids and oral corticosteroids combined with vitamin D(3) on the early clinical and immunological effects of SIT. Methods It was a randomized, double-blind, placebo-controlled trial conducted in 54 asthmatic children allergic to house dust mites. Intervention was based on receiving a single dose of oral steroid, with or without vitamin D(3), or placebo only on the day of the build-up phase of SIT. Results After 12 months of SIT, the median daily inhaled corticosteroid (ICS) dose, which controls the symptoms of asthma, was reduced by 25% in the steroid group. However, a 50% reduction of the median daily ICS dose was observed in the control group. The clinical effects of SIT were not affected in the steroid+D(3) group. Concomitantly, we found that intervention with prednisone significantly impaired the induction of T regulatory lymphocytes. Importantly, the clinical and immunological effects of SIT were not affected by intervention with steroids administered with vitamin D(3). Conclusions Our study failed to show a beneficial effect of oral corticosteroids on allergen-specific immunotherapy. We observed that the combined administration of a corticosteroid drug and allergen extract suppressed the early clinical and immunological effects of SIT and that vitamin D(3) prevented this ‘adverse’ influence of steroids.

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/ title=”Magnesium and selected parameters of the non-enzymatic antioxidant and immune systems”>
Magnesium and selected parameters of the non-enzymatic antioxidant and immune systems

January 2015

INTRODUCTION: The present study investigated associations between environmental exposure to magnesium (Mg) and the levels of oxidative stress parameters and selected cytokines, and the antioxidant defense system in the seminal plasma of fertile males. MATERIALS AND
METHODS:
The study population consisted of 57 healthy, non-smoking, fertile men from the southern region of Poland. Based on the median magnesium levels in seminal plasma, subjects were divided into two groups: those with low (Mg-L) and high (Mg-H) magnesium concentrations.
Results:
Differences were not observed between the Mg-L and Mg-H groups as regards semen volume, pH, count, motility, or morphology of sperm cells. In the Mg-H group, cholesterol levels were significantly higher (77%) compared with the Mg-L group: however,?-glutamyltranspeptidase activity was significantly lower (21%). The values of total oxidant status were significantly different between the two groups, with 78% higher values observed in the Mg-H group compared with the Mg-L group, whereas malondialdehyde levels did not differ significantly. The values for total antioxidant capacity and uric acid levels were significantly lower in the Mg-H group compared with the Mg-L group (14% and 17%, respectively). However, levels of bilirubin, albumin, thiol groups and ?-tocopherol were significantly higher in the Mg-H group (71%, 44%, 35% and 47%, respectively).
Conclusion:
No associations between Mg levels in the seminal plasma of fertile males and standard semen parameters were found. However, Mg levels may be associated with altered function of the non-enzymatic antioxidant system.

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/ title=”Antimicrobial peptides, innate immunity, and the normally sterile urinary tract.”>
Antimicrobial peptides, innate immunity, and the normally sterile urinary tract.

November 2007

Considering the anatomical location of the urethral meatus, it is surprising that urine is normally sterile. The defensive properties of uroepithelia help maintain this sterility as strategically necessary for long-term survival. Epithelia lining the urinary tract prevent adhesion of bacteria by release of Tamm-Horsfall protein, lactoferrin, lipocalin, and constitutive and inducible bactericidal antimicrobial peptides such as alpha- and beta-defensins and cathelicidin. Microbes that overwhelm these early defenses contact uroepithelia and activate an innate immune response through Toll-like receptor 4. With persistence of increasing numbers of microbes, chemokines (IL-8) and cytokines (IL-1 and TNFalpha) attract and activate large numbers of neutrophils and macrophages that damage tubulointerstitial parenchyma. The risk of serious infection in humans seems quite variable. Cathelicidin, for example, is a vitamin D-dependent gene, and vitamin D stores may influence susceptibility to urinary tract infection in selected individuals. As more knowledge accrues, vitamin D supplementation may someday be useful as adjuvant therapy in this setting.

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/ title=”Effects of rooibos tea extract on antigen-specific antibody production and cytokine generation in vitro and in vivo.”>
Effects of rooibos tea extract on antigen-specific antibody production and cytokine generation in vitro and in vivo.

October 2001

Rooibos tea contains a large amount of flavonoids and acts as a potent antioxidant. In this study, we examined the effects of Rooibos tea extract on antigen-specific antibody production and cytokine generation in vitro and in vivo. The primary in vitro anti-ovalbumin (anti-OVA) or sheep red blood cell (SRBC) antibody production in murine splenocytes was markedly stimulated by the addition of the tea extract at concentrations of 1-100 microg/ml. On the other hand, a nonspecific antibody response elicited with lipopolysaccharide (LPS) in purified splenic B-cells was not modified by the extract. Rooibos tea extract caused an increase in the generation of interleukin 2 (IL-2) both in OVA- and anti-CD3-primed splenocytes at concentrations ranging from 10 microg/ml to 1000 microg/ml. In contrast, this tea extract suppressed the generation of interleukin 4 (IL-4) in OVA-primed splenocytes. Moreover, the reduction of OVA-induced antibody production in serum of the cyclosporin A (CyA) -treated rats can be significantly restored and the IL-2 generation in murine splenocytes was stimulated, following oral administrations of Rooibos tea extract. Thus, our findings suggested that Rooibos tea extract may facilitate the antigen-specific antibody production through selective augmentation of IL-2 generation both in vitro and in vivo. Collectively, Rooibos tea intake may be of value in prophylaxis of the s involving a severe defect in Th1 immune response such as cancer, allergy, AIDS, and other infections.

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/ title=”Cardio-metabolic and immunological impacts of extra virgin olive oil consumption in overweight and obese older adults a randomized controlled trial.”>
Cardio-metabolic and immunological impacts of extra virgin olive oil consumption in overweight and obese older adults a randomized controlled trial.

December 2014

Background:
Both aging and obesity are related to dysregulated immune function, which may be responsible for increased risk of infection and also chronic non-infectious s. Dietary lipids have been shown to impact immune and inflammatory responses and cardio-metabolic risk factors. No information on the impact of olive oil on immune responses of overweight and obese older adults is available. OBJECTIVE:
We aimed to determine the effect of replacing oils used in a typical American diet with extra virgin olive oil for 3 months on immune responses and cardio-metabolic risk factors in overweight and obese older adults.
METHODS:
This was a randomized, single-blinded and placebo-controlled trial in 41 overweight or obese participants (aged???65) who consumed a typical American diet. Participants in the control (CON, n?=?21) group were provided with a mixture of corn, soybean oil and butter, and those in the olive oil (OO, n?=?20) group, with extra virgin olive oil, to replace substitutable oils in their diet. At baseline and 3 months, we measured blood pressure, biochemical and immunological parameters using fasting blood, and delayed-type hypersensitivity (DTH) skin response.
Results:
Compared to the CON group, the OO group showed decreased systolic blood pressure (P?

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